Semiconductor Nanowires Biosensors for Highly Selective and Multiplexed Detection of Biomolecules

Tijjani Adam, Aeshah Muhana Mohammed, Th. S. Dhahi, Nur Fatimi Nor Azua, N. Azizah, U. Hashim, Subash C.B. Gopinath


The surface modification of Nano-structure has allowed specific and selective detection to be made on nano structures devices. Current study, a nanowire was surface engineered with the potential of silicon nanowires biosensors (SiO2) which enhance the biosensor activity especially identifying single-stranded bio-molecular such as E.coli DNA. The device's capabilities were studied based on it response n electrochemical activities of the terminal group of the surface modification agent. NH2 -terminated APTES) to provide rigid chemistry between the DNA organic and Si inorganic link of a biomolecule single_stranded ssDNA probe and SiO2_APTES link nanostructure. Thus, the study demonstrates that silicon nanowire sensing capability to discriminate molecular probe to that of molecule target of supra-genome 21 mers salmonella due to sensitive surface chemistries that made distinguishing the two species. The device captured the molecule precisely; the approach took the advantages of strong binding chemistry created between APTES and biomolecule. The results indicated how modifications of the nanowires provide sensing capability with strong surface chemistries that can lead to specific and selective target detection.


APTES; DNA Biosensor; E-coli; Multiplexed Detection;

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Guo-Jun Zhang and Yong Ning (2012). Silicon nanowire biosensor and its applications in disease diagnostics: A review.Analytical Chemical Acta 749 pp 1–15

Guo-Jun Zhang, Li Zhang, Min Joon Huang, Zhan Hong Henry Luo, Guang Kai Ignatius Tay, Eu-Jin Andy Lim, Tae Goo Kang, Yu Chen (2010) Silicon nanowire biosensor for highly sensitive and rapid detection of Dengue virus, Sensors and Actuators B: Chemical Vol. 146 (1), pp138–144

J, L., Y, Z., S, T., L, Y., & Y, S. (2011). Effect of nanowire number, diameter, and doping density on nano-FET biosensor sensitivity. ACS Nano , 1-8.

Katz E, Willner I. Biomolecule-functionalized carbon nanotubes: applications in nanobioelectronics. ChemPhyschem. 2004;5 : 1084- 1104

Kuan-I Chena, Bor-Ran Lia, Yit-Tsong Chen (2012) Silicon nanowire field-effect transistor-based biosensors for biomedical diagnosis and cellular recording investigation, Nano Today. Vol 6(2) pp 131–154

L. De Vico, L. Iversen, M.H. Sorensen, M. Brandbyge, J. Nygard, K.L. Martinez et al. (2011). Predicting and rationalizing the effect of surface charge distribution and orientation on nano-wire based FET biosensors, Nanoscale.3, pp.3635–3640

Li Y, Afrasiabi R, Fathi F, Wang N, Xiang C, Love R, et al. Impedance based detection of pathogenic E. coli O157:H7 using a ferroceneantimicrobial peptide modified biosensor. 2014. p. 193–9. View Article PubMed/NCBI Google Scholar

Li Y, Cheng P, Gong J, Fang L, Deng J, Liang W, et al. Amperometric immunosensor for the detection of Escherichia coli O157:H7 in food specimens. Analytical Biochemistry. 2012;421 (1):227–33. doi: 10.1016/j.ab.2011.10.049. pmid:22119072 View Article PubMed/NCBI Google Scholar

Lin Y-H, Chen S-H, Chuang Y-C, Lu Y-C, Shen TY, Chang CA, et al. Disposable amperometric immunosensing strips fabricated by Au nanoparticles- modified screen?printed carbon electrodes for the detection of foodborne pathogen Escherichia coli O157:H7. Biosensors and Bioelectronics. 2008;23 (12):1832–7. doi: 10.1016/j.bios.2008.02.030. pmid:18424027 View Article PubMed/NCBI Google Scholar

Tijjna Adam U. Hashim and Th S. Dhahi, Silicon nanowire formed via shallow anisotropic etching, Si-ash-trimming for specific DNA and electrochemical detection, Chinese Physics B Vol. 24, No. 6 (2015) 06810

Tijjani Adam, U Hashim, Highly sensitive silicon nanowire biosensor with novel liquid gate control for detection of specific single-stranded DNA molecules, Biosensors and Bioelectronics. 67(2015) 656–661

Md. Eaqub Ali, Th.S. Dhahi, Rasel Das, U. Hashim, DNA hybridization detection using less than 10-nm gap silicon nanogap structure, Sensors and Actuators A 199 (2013) 304–309.

Chen, X., Guo, Z., Yang, G.-M., Li, J., Li, M.-Q., Liu, J.-H., & Huang, X.-J. (2010). Electrical nanogap devices for biosensing. Materials Today, 13(11), 28-41. doi

M Wesam Al-Mufti, U Hashim, M Rahman, Tijjani Adam, M Arshad, Studying Effect Dimensions of Design and Simulation Silicon Nanowire Filed Effect Biosensor, Applied Mechanics and Materials 754, 854-858, 2015.

Yi, M., Jeong, K.-H., & Lee, L. P. (2005). Theoretical and experimental study towards a nanogap dielectric biosensor. Biosensors and Bioelectronics, 20(7), 1320-1326

Uda Hashim, N. T., Thikra S. Dhahi, Azizullah Saifullah. (2011). Polysilicon nanogap structure development using size expansion technique. Microelectronics International, 28(3), 24-30

Wang, C., Huang, J., Wang, J., Gu, C., Wang, J., Zhang, B., & Liu, J. (2009). Fabrication of the nanogapped gold nanoparticles film for direct electrical detection of DNA and EcoRI endonuclease. Colloids and Surfaces B: Biointerfaces, 69(1), 99-104

Yexian Wu, T. A., Sebastian Gautsch, Nico De Rooij. (2011). Development of passivated Heteregeneous metal nanogaps using EBeam Overlay Technique. Paper presented at the Proc. Eurosensors XXV, Anthens, Greece.

Francois Beguin, E. F. (Ed.). (2013). Supercapacitors, Materials,Systems, and Applications (Vol. 4). Germany: Wiley-VCH Verlag GmbH & Co.

Dimitrios P.Nikolelis, T. V., Arzum Erdem, Georgia-Paraskevi Nikoleli (Ed.). (2014). Portable biosensing of food toxicants and environmental pollutants (Vol. 1). New York: CRC Press.

Tijjani Adam, U Hashim, Design and fabrication of micro-mixer with short turns angles for self-generated turbulent structures, Microsystem Technologies, 1-8, 2015

T Adam, U Hashim, TS Dhahi, KN Khor, PS Chee, PL Leow, ELECTROCHEMICAL ETCHING: An Ultrasonic Enhance Method of Silicon Nano Porous Fabrication, Wulfenia Journal 20 (1), 45-55, 2013

U Hashim, T Adam, J Lung, P Ling, Fabrication of Microchannel and Micro Chamber for Microfluidic Lab-on-Chip.Australian Journal of Basic & Applied Sciences 7 (1)2013

T Adam and U. Hashim Light Observation in Polymer: A Study of Silicon-Based Organic Polymer Using Visible Spectroscopy, Australian Journal of Basic and Applied Sciences 7 (1), 76-80, 2013.

U Hashim, MW Al-Mufti, T Adam, Silicon Nanowire Geometry: Investigation of Interaction Site Potential in Semiconductor-DNA Interaction, Australian Journal of Basic and Applied Sciences 7 (5), 242-245, 2013

MW Al-Mufti, U Hashim, T Adam, Simulation of Nano lab on chip devices by using COMSOL MultiphysicsJournal of Applied Sciences Research 9 (2), 1056-1061, 2013

T Adam, U Hashim, KL Foo, TS Dhahi, T Nazwa, Technology development for nano structure formation: Fabrication and characterization, Advanced Science Letters 19 (1), 132-137, 2013

T Adam, U Hashim, PL Leow, KL Foo, PS Chee, Selection of optimal parameters in fabrication of poly (dimethylsiloxane) microfluidics using taguchi method, Advanced Science Letters 19 (1), 32-36,2013

T Adam, U Hashim, KL Foo, Microfluidics design and fabrication for life sciences application, Advanced Science Letters 19 (1), 48-53, 2013

T Adam, U Hashim, ME Ali, PL Leow, The electroosmosis mechanism for fluid delivery in PDMS multi-layer microchannel, Advanced Science Letters 19 (1), 12-15, 2013


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