Automatic Gram Staining for Sputum Slide

K.P. Ping, Rosyati Hamid, Faradila Naim, Nurul Wahidah Arshad, Zeehaida Mohamed


The Gram stain is the most important and universally used staining technique in the bacteriology laboratory. Gram staining method is used to do staining of the clinical material or the bacteria from colonies on laboratory media and provide a direct visualization of the morphology of the organisms based on their reactions to the chemical present in stains. A sputum sample slide need to be stained before the quality of the sputum sample is determined. However, due to human inconsistency, some of the slides are heavily stained with dark color whereas some of it is lightly stained. This inconsistency would create a difficulty for automated sputum quality system using image processing. Therefore, an automated gram staining for sputum slide is needed in order to standardize the slide staining. The automated Gram-staining will undergo staining, washing, and drying process. Each process periods are controlled by a timer built in the microcontroller. The analysis is done on the accuracy of the position of the slide stain, the consistency of the amounts of staining solution drop on the slide and the time efficiency of this automated system is compared to manual operation.


Gram Staining; Sputum; Automated System;

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W. Lawrence Drew, Andres N. Pedersen And Jacques J. Roy. 1971. Automated Slide Staining Machine. Applied Microbiology. IEEE, 23(1): 17-20.

O. A. N. Husain, J. M. Grainger And J. Sims. 1978. Cross contamination of cytological smears, with automated staining machines and bulk manual staining procedures. Journal of Clinical Pathology. 31: 63-68

Felek, S. and Arslan, A. 1999. Gram Staining with an Automatic Machine. Folia Microbiology. 44(3): 333-337.

River CW Wong, MPhil, Samuel SY Heung, MSc, YC Ho, MSc, KT Yip et al. 2010. Evaluation of PREVI Color Gram Automated Staining System on Positive Blood Culture Samples. LABMEDICINE. IEEE, 42(7).

Ellen Jo Baron, Samantha Mix And Wais Moradi. 2009. Clinical Utility of an Automated Instrument for Gram Staining Single Slides. Journal of Clinical Microbiology. 48(6): 2014.

Soo Chan Kim, Seung Il Kang, Deok Won Kim, Seung Cheol Kim, Sang-Nae Cho, Jung Ho Hwang, Young Kim, Sun-Dae Song, Young Ha Kim. 2003, Development and evaluation of an automated stainer for acid-fast bacilli. Medical Engineering & Physics. IEEE, 25: 341–347.

Nur Shahida Nawi, Rosyati Hamid, Nurul Wahidah Arshad, Faradila Naim, Mohd Falfazli Jusof, Mohd Najib Razali. 2013. The Detection and Summation of Squamous Epithelial Cells for Sputum Quality Testing. Faculty of Electrical & Electronics Engineering, Universiti Malaysia Pahang, IEEE, 978-1-4673-6195-8/13,2013.


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ISSN: 2180-1843

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